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By Alton Meister

Advances in Enzymology and similar parts of Molecular Biology is a seminal sequence within the box of biochemistry, supplying researchers entry to authoritative reports of the newest discoveries in all components of enzymology and molecular biology. those landmark volumes date again to 1941, offering an unmatched view of the historic improvement of enzymology. The sequence bargains researchers the newest knowing of enzymes, their mechanisms, reactions and evolution, roles in advanced organic technique, and their program in either the laboratory and undefined. each one quantity within the sequence beneficial properties contributions through prime pioneers and investigators within the box from around the globe. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.

With its wide selection of subject matters and lengthy historic pedigree, Advances in Enzymology and similar parts of Molecular Biology can be utilized not just by means of scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition by means of any scientist drawn to the invention of an enzyme, its homes, and its applications.

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I X . Other Imidazole Compounds . . . . . . . . . . . . . . . . . . X . Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . 51 . . . . . 52 53 53 58 58 60 61 61 63 63 65 66 67 67 69 75 76 76 79 81 82 82 86 87 89 91 91 92 92 94 95 96 96 98 99 100 100 101 101 103 103 52 E. A. BARNARD AND W. D. STEIN I. , there will be for any side-chain group certain situations where the effect on the protein function of its presence will be decisive or a t least significant, although elsewhere it has only an ancillary role and can be replaced by other groups within certain limits.

Such experiments would be sufficient LIVER ALCOHOL DRHYDROGENASR 45 to give correct data for the velocity constants, if our postulated mechanism, equations (1) through (3), were valid, but insufficient to disclose the origin of deviations from this niechnaism. For example, as seen from equations (4) and ( 5 ) , it is assumed that the maximal velocity, Vm,,/e,is equal to Icz’ in equation (4) and lcz in equation (5) ; these are the dissociation velocity constants of D P K - A D H and DPNHvADH. However, kt appears in the last term of equation (4) as well, and ki in equation ( 5 ) .

G . A Note on A V Hydrogen Bonding . . . . . . . . . . . . . . . . . . . . . . A . Evidence for Hydrogen Bonding . . . . . . . . . B Carbohydrate-Splitting Enzymes . . . . . . . . . C . Permeability Systems . . . . . . . . . . . . . . . . . . VI . Imidazole-Metal Interactions . . . . . A Protein Metal Binding . . . . . . . . . . . . . . . . . . B. Metals within a Prosthetic Group .

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